The mechanism of triosephosphate dehydrogenase inactivation by reduced diphosphopyridine nucleotide.
نویسندگان
چکیده
We have shown in a previous communication (1) that the enzyme triosephosphate dehydrogenase is rapidly inactivated in the presence of reduced diphosphopyridine nucleotide at physiological concentrations, temperature, and pH. Good stoichiometry was shown for the amount of enzyme inactivation, DPNH-X1 formation, and disappearance of sulfhydryl groups. It was then of particular interest to determine the detailed mechanism of the inactivation phenomenon and whether or not these indicated changes were truly related. As shown in this paper, the mechanism of DPNH-induced enzyme inactivation has been clarified, and DPNH-X formation and enzyme inactivation can be differentiated.
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 237 شماره
صفحات -
تاریخ انتشار 1962